A: percent GFP-positive cells calculated using the classification feature in the software. Download SoftMax® Pro 7. . The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring Download SoftMax® Pro 7. Wokingham, Berkshire RG41 5TS United Kingdom. The dynamic range of the assay is about 3. Expanded dynamic range Engineered for performance with spectral fusion illumination for increased sensitivity across the entire excitation range. SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. Title: Fluorescence Intensity SpectraMax Paradigm or i3 Detection Cartridge | Molecular Devices Author: Molecular Devices, LLC Subject: Explore this datasheet to know more about Fluorescence Intensity Detection Cartridges for SpectraMax Paradigm and i3x, which provide speed, sensitivity, and flexibility & uncompromising performance for either top or. 0´ 105 dsD NA (p g/ wel ) R F UFluorescence intensity was measured using a Spectramax i3x plate reader (Molecular Devices) with excitation at 575 nm (bandwidth 9 nm) and emission at 608 nm (bandwidth 15 nm). ai; ImageXpress Pico; IN Carta Image Analysis Software; CLONE SCREENING. 99. . 306 plate air cal fail,low light. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. FlexStation® 3 Multi-Mode Microplate Reader. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. ZERO BIAS - scores, article reviews, protocol conditions and more. . At each time point, the samples were shaken and fluorescence was measured for SEP (488-nm excitation and 530-nm emission) and. EMax Plus Microplate Reader. 304 signal level saturation. Spectramax I3x, supplied by Danaher Inc, used in various techniques. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. . Molecular Devices Software. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. SpectraMax i3/i3x readers must be equipped with a suitableThe SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. . ; Page 2 Molecular Devices equipment, software, reagents, and consumables. The plate was shaken and incubated in the dark for 10 minutes. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析 We would like to show you a description here but the site won’t allow us. Find regional support. which chemiluminescence was detected on a SpectraMax i3x reader (Molecular Devices) and analyzed us ing GraphPad. Quick imaging and analysis of cells gives you a front-row view of the phenotypic changes that accompany. . Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. In this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. . Designed specifically for the SpectraMax® i3 Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick inspection of cells and provides insight into phenotypic changes that are important for understanding cytotoxicity, cell proliferation, and protein expression. CHO M1WT3 cells are Chinese hamster ovary cells stably transfected with the M1 muscarinic G q -coupled receptor. 4. 100 fmol 384-well (75 µL) 0. Find regional support. Curves were forced through x,y = 0. The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform that offers cellular imaging capability. SoftMax Pro User’s Manual v Contents Figures. SpectraMaxParadigmMulti-ModeDetectionPlatformUserGuide 4 5014038F Chapter5:ReadModesandReadTypes 71 SupportedReadTypes 71 AbsorbanceReadMode 74 FluorescenceIntensityReadMode 79the SpectraMax Paradigm Multi-Mode Detection Platform User Guide. モジュールを追加してアップグレードすることにより、ウェスタンブロットやイメージング、試薬インジェクタを用いた高速カイネティック測定を行う. . SpectraMax Microplate Readers. Explore Multi-Mode Validation Plate that confirms instrument performance for spectramax i3, i3x, Paradigm system & FilterMax Reader. Quantiferon-TB Gold in Tube Kit. Eliminate time-dependent substrate addition steps. Videos & Webinars. . Materials and methodsSpectraMax i3x Multi-Mode Microplate Reader. More Product Information. 1、卡盒式模块和细胞成像模块的构架检测功能（如FP、TRF、HTRF、AlphaScreen、ScanLater Western Blot等）卡盒模块化设计，仅通过插入卡盒就能可. Spectranet customer portal. Spectramax I3x, supplied by Danaher Inc, used in various techniques. When used in combination with the SpectraMax Injector Cartridge, this system enables a wealth of flash type luminescence applications including reporter gene and other assays with exceptional sensitivity and dynamic range. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeSpectraMax i3x and iD5 readers can be equipped with an HTRF detection cartridge or Enhanced TRF module and filters, respectively. . See Unpacking the SpectraMax Paradigm Instrument on page 1. 58% for the detection module and 2. Time-. were measured at OD405 using a SpectraMax i3x multimode detection system (Molecular Devices, San Jose, CA, USA), and the melanin concentration was determined using the standard curve of melanin solutions with known concentrations. Danaher Inc spectramax i3x. The SpectraMax i3x reader provides the. Published Dec 16, 2020 | Updated Oct 10, 2023. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. 13 There are a fairly limited number ofSARS-CoV-2 variants of concern (VOCs) that increase transmission or disease severity or reduce diagnostic or vaccine efficacy continue to emerge across the world. Enzyme Activation Analysis;. SPECTRAMAX i3X, INJECTOR MODULE CARTRIDGE model number 0200-7029. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. 1. 01 0. For wavelengths in the UV range above 220 nm,. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Price from $9. 100 fmol 1536-well (8 µL) 0. User-upgradeable detection modules allow you to expand the capabilities of your microp. Specifically, we present neurite. Results for SpectraMax i3x reader with NanoBRET Detection Module (blue) and on-board luminescence detection mode (orange). 1-xiAt last, the relative absorption was estimated using a microplate reader (SpectraMax i3x, Molecular Devices, LLC, Sunnyvale, CA, USA) at 450 nm. Note: Additional readers with similar performance are listed. SpectraMax iD3 and iD5 Register or Sign In. Core features include biological and fume hoods, a cold room and multiple freezers, and access to institutional space for radiolabeling and radiation monitoring. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Sensitive TRF detection of two simple, mix-and-measure assay workflows. Ease of use. Controls containing all reaction. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. (SpectraMax i3/i3x) CatchPoint SimpleStep ELISA (SpectraMax Paradigm) SpectraDrop. application modules • Multi-mode microplate reader and imaging system • Wide dynamic range • StainFree™ cell counting technology • Library of user-configurable applications • Advanced curve fitting and statistical analysis • Cell confluence and cell viabilitySoftMax® Pro 7. Italic and boldface type are used for emphasis. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. In this product video, see how the SpectraMax M5 quickly reads samples. Data analysis. Figure 1. to View Figures. Rutin was used to generate standard curves for quantification. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. ClonePix 2 Mammalian Colony Picker; QPix Microbial Colony Pickers; CloneSelect Imager; DispenCell Single-cell Dispenser; 3D. R8362. For researchers working in GLP or GMP laboratories, achieve full GxP compliance with our turnkey solution:. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. View the Molecular Devices website. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . 0 mm hex key to remove the six blue retaining screws that secure the transport bracket (B) to the instrument. Modularity does provide some measure of forward vision into upcoming or future experimental needs. Microplate Spectrophotometers. Ease of use Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. No accessories are needed and SoftMax® Pro. Bioz Stars score: 98/100, based on 1 PubMed citations. The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. The assay can accurately test the toxicology of compounds as shown in Figure 1. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013. 2. The TUNE detection cartridge uses a breakthrough monochromator design that enables researchers to easily optimize their. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree Technology Measure oxidative metabolism and glycolytic activity on the SpectraMax i3x reader. Monday to Friday 7am - 5pm Pacific Time. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideDiscover how the automation-friendly SpectraMax® i3x Multi-Mode Microplate Reader, designed to expand research capabilities through its versatile and user-upgradeable application modules, can. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence polarization, and time-resolved. The M5 can read volumes down to 2uL with the uMax Low. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. applications, including dual luciferase and ATP assays. 2/5 apart, not needed o Otherwise, ensure cutoff is 10nm less than desired emission wavelength. Introduction Inflammation, the body’s reaction to infection, irritation, or other injury, is accompanied by increased endothelial chemokine. Read more about HTRF SpectraMax Detection Cartridges for SpectraMax i3x & Paradigm Systems which utilize a high energy Xenon flash lamp for the sensitive reading of HTRF reagents. gen) on a Spectramax i3X luminometer. Actual shake speed is based on the microplate format. Danaher Inc spectramax i3x. 030 fmol. +1 800-635-5577. Get a front row view of your cells. com. com. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. Molecular Devices SpectraMax i3x Multi-Mode Detection Platform. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. Catalog excerpts. The information is below the horizontal line. Published Dec 16, 2020 | Updated Apr 13, 2023. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. ClonePix 2 Mammalian Colony Picker; QPix Microbial Colony Pickers; CloneSelect Imager; DispenCell Single-cell Dispenser; 3D. o For example: Ex: 485/Em 525/Cutoff 515 4. The soil suspension (600 µL) and the substrate (L-3,4-dihydroxyphenylalanine, DOPA, 150 uL, 25 mmol L -1 ), were mixed and then added to. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. 1. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. g. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. 303 unable to cal dark current. 2 nM was calculated using SoftMax Pro Software. Microplate optimization should be performed the first time the assay is run, and subsequently for . 312 gain calibration failed. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellThis characterization of nanoparticle and its surface interactions can be detected using the SpectraMax i3x Multi-Mode Microplate Reader and the Spectral Optimization Wizard in SoftMax Pro Software. . Viewing this answer requires an authorized account. SpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. The Results. Detection cartridge modularity allows for easy addition of new read modes within minutes without. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . i3. Future ready The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular. Reader spectramax microplate id3 molecular devices multi mode boundaries functionality instrumentSpectramax i3x multi-mode microplate reader with minimax imaging Molecular devices spectramax i3x 使用される 販売のために 価格. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays, enzyme. The SpectraMax M4 reader had identical performance specifications to the SpectraMaxLuminescence was read on the SpectraMax i3x reader and the SpectraMax L reader. . Itemploysa. com. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. . 2 Parallel Line Analysis Conc log [ng/ML] RLU. Table 1. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Open the catalog to page 2. SpectraMax® i3x. Unparalleled performance on a personalized platform. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. SpectraMax i3x Register or Sign In. . Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Pick the best speed, sensitivity, and price for your needs. Spectramax I3x, supplied by Danaher Inc, used in various techniques. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application. Use the 3. Find regional support. Both Microplate Optimization and Read Height Adjustment were. Expanded dynamic range The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. After 2 hours of treatment cells were harvested by centrifugation and cell pellets were lysed in RIPA buffer (20mM Tris-Cl, 150mM NaCl, 0. ”Figure 3: Sensitivity of the SpectraMax i3x reader in detecting increasing dilutions of the MycoAlert Assay Control. 10. Price from $9. +44-118-944-8000. . Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. Monochromator optics with adjustable bandwidth offer excellent sensitivity down to 6 nM tryptophan, as well as precise determination of the fluorescence emission peak shift that occurs upon protein denaturation. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. SpectraMax Microplate Reader - Choose from our extensive SpectraMax® line of user-friendly microplate readers. Fractional occupancy Corrected BRET ratio NanoBRET. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. EUROPE. . Contact Local Team. Nuclear Receptor. The corrected absorbance is within 5% of the. Capture Flash Assays with Ease. Nucleic Acid and Protein Quantitation. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. 2 only) SpectraMax M-series Microplate readers. ImageXpress Confocal HT. Published Dec 16, 2020 | Updated Dec 16, 2020. 3. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. 1. 4. StakMax Handling Stacker Base Unit. 2 Parallel Line Analysis Conc log [ng/ML] RLU. The protocols are also available on the SoftMax Pro Software protocol sharing. Molecular Devices推出的SpectraMax® i3x 多功能酶标仪除了具有全波长的光吸收、荧光、化学发光和FRET检测功能以外，还兼容了客户端模块化升级功能，用户可以根据需要任何时间随意升级至荧光偏振FP、HTRF、AlphaScreen、Western Blot、上转发光检测、细胞成像和带有注射器. Note: Additional readers with similar performance are listed. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. We would like to show you a description here but the site won’t allow us. The SpectraMax DuoLuc Reporter Assay has been performance optimized on the SpectraMax i3x reader with Injector Cartridge. 1. Find our technical support information along with contact details. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. ZERO BIAS - scores, article reviews, protocol conditions and moreThe SpectraMax ® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and. Expand your lab’s capabilities to include flash applications such as dual luciferase and. The Immunochemical Core includes approximately 2,600 square feet of space in the Joseph Building at Mayo Clinic Hospital, Saint Marys Campus. Explore a wealth of applications with the future-ready SpectraMax i3x. . Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. The solutions were thoroughly mixed and then incubated in the dark at 20°C for 2 h. Replicate values were averaged and imported into Design-Expert 8. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. SpectraMax i3x Multi-Mode Microplate Reader; SpectraMax MiniMax 300 Imaging Cytometer *Here, the NucView 488 with DMSO formulation was used. Asterisks indicate the dilution of assay control that should be minimally detectable for each kit. My proficiency in techniques such as •PBMC isolation, counting and. . ImageXpress Confocal HT. MC38 in vivo tumor model in hPD-1. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 313 reference gain check fail. to View Figures. Fluorescent Polarization: Solid black plates, as above. The Curcumin binding to p53Y220C was also measured using the emission maxima of Curcumin at 495 nm after. . Prepare your i3/i3x instrument for shipment. Flash-type luminescence with excellent sensitivity Expression measurement in as few as ten cells per well Improved accuracy of results by. were performed on a Biomek i7 containing an integrated orbital plate shaker and a SpectraMax i3x plate reader (Molecular Devices). As per Volza’s India Import data, Spectramax import shipments in India stood at 365, imported by 145 India Importers from 28 Suppliers. . Select PMT gain o PMT gain = energy used by detector to collect signal o Low gain for samples with high expected signalCapture flash assays with ease The SpectraMax i3x Injector Cartridge with SmartInject® Technology expands your research capabilities to include flash-based applications including dual luciferase and ATP assays. Shaking and incubations were performed using an on-deck orbital shaker. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. User-upgradeable detection modules allow you to expand the capabilities of your microp. Spectranet customer portal Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. For each kit, the reader was able to detect the lowest dilution as positive. The chamber. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. SpectraMax i3x Multi-Mode Microplate Reader. Molecular Interactions. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 1 and above:. The SpectraMax Paradigm Multi-Mode Detection Platform is a modular multi-mode microplate detector. Concentrating the excitation light on the sample through label specific optics, high levels of sensitivity and performance are reached. SpectraMax i3x; SpectraMax iD3 / iD5; SpectraMax M Series; SoftMax Pro Software; CELLULAR IMAGING. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. As an aspirant Bioscience research with expertise in cell biology, immunology and bio instrumentation techniques, I bring a wealth of knowledge and expertise to the table. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Keywords: filtermax multi mode microplate readers, spectramax paradigm multimode detection platform, spectramax paradigm system Created Date: 11/16/2010 12:21:51 PMThe SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. Tristar 5 Multimode Microplate Reader. #0200-7009) Contact our support team with your questions regarding Molecular Devices products and applications. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. . The NET-specificity of the detected signal was determined by incubation with DNase I (50 U/mL, Qiagen, Germany). Unplug the power cable from the back of the instrument. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. . Page 9 Setting Up the SpectraMax® MiniMax™ 300 Imaging Cytometer 2. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Figure 2-23: Bottle holder To install the bottle holder: 1. Explore a wealth of applications with the future-ready SpectraMax i3x. We would like to show you a description here but the site won’t allow us. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploringfluorescence polarization, trf, fret, igg quantitation, elisa, western blot, spectramax i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiterA microplate reader that can detect two or more applications is considered a multi-mode plate reader. All the readers were operated using SoftMax® Pro Software, which also has built-in data analysis and curve-fitting for automatic calculation of EC 50. The SoftMax Pro Software provides integrated instrument control. 吸収度マイクロプレートリーダー SpectraMax® Mini. With a preconfigured protocol to simplify data acquisition and analysis, SoftMax Pro Software completes this total solution for fast, sensitive, and reliable dual luciferase reporter assay results. 2-µL or 4-µL samples were pipetted onto a 64-well SpectraDrop slide in quadruplicate, following the plate map in Figure 2. Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. Setting iD5 i3x M5 Paradigm Measurement mode Fluorescence Polarization Fluorescence Polarization Fluorescence Polarization Fluorescence Polarization Excitation 485 nm monochromator 485 nm 485 nm 485 nmPage 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Figure 1. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. . Molecular Devices are known for both the reliability and the wide uses of their products. Shop For Laboratory Equipment featuring a wide variety of new, used and refurbished laboratory equipment. 001 0. The SpectraMax i3x reader provides the The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. compound concentration, and an IC 50 value of 21. 3. Hold down the green power button on the front of the instrument for 5 seconds or until the instrument power down. Introduction. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. In this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 1. Danaher Inc spectramax i3x. As shown in the results above, the DELFIA cell proliferation assay combined with the SpectraMax i3 reader gives researchers a very sensitive test for measuring cell proliferation. The CellTiter-Glo assay from Promega uses luciferase. Settings not required by a reader are indicated by ‘---’. 0. . The SpectraMax i3x reader allows you to unravel the mysteries of science by exploringLuciferase activity was measured using a SpectraMax i3x microplate reader (Molecular Devices). 3: The cuvette chamber. 5mM EDTA, 1% NP40,Explore a wealth of applications with the future-ready SpectraMax i3x. The e ect of ASC-exosomes on the melanin levels inThe 70% methanolic extract of steamed root (100 µL) was added to 1 mL of diethylene glycol and 10 µL of sodium hydroxide (NaOH). SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. The 4x washing steps were also performed on deck using the Span-8 aspirate and dispense for the first three washes. 003 fmol 0. 315 can't find zero order. Learn how you can perform flash-based applications, such as dual luciferase and ATP assays, with high precision and accuracy using this cartridge. However, no piece of equipment is immune to breaking down. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Prepare your iD series instrument for shipment. Dilution series of HeLa cells transfected with firefly (red plot) and Renilla (blue plot) luciferases. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. 2. Citation 2002). Example: [Drawer]. The SpectraMax® i3 Multi-Mode Microplate Reader Detection Platform evolves with your future research needs and offers a breadth of application possibilities. The user guide also covers the features and specifications of the SpectraMax MiniMax 300. i3x. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. Restart the system. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. ，相关视频：多功能酶标仪SpectraMax i3x的维护操作-注射器卡盒，多功能酶标仪SpectraMax iD5的维护操作-注射器卡盒，多功能酶标仪SpectraMax i3x的维护操作-卡盒的安装和拆卸，细胞计数及活率分析仪 Vi-CELL BLU 功能&操作讲解，CRISPR基因编辑系统介绍：Cas9和Cas12a，流式.